Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDGVMILPVLIMIALPSPSMEDEKPKVNPKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLACLLGVALRKFKRRNQERLNPRDVEYGTIEGLITTNVGDSTLADLLDHSCTSGSGSGLPFLVQRTVARQITLLECVGKGRYGEVWRG---SWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGF--IASDMTSRHSSTQLWLITHYHEMGSLYDYLQLTTLDTVSCL-RIVLSIASGLAHLHIEIFGTQGKPAIAHRDLKSKNILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVSNGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRP---NIPNRWFSDPTLTSLAKLMKECWYQNPSARLTALRIKKTLTKIDNSLDKLKTDC 6OKO Chain:A ((14-271)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VP-LVSR---EELKKLEFVGKGGFGVVFRAHHRTW-NHDVAVKIVNSK-KISW----EVKAMVNLRNENVLLLLGVTEDLQWDFVSGQA-LVTRFMENGSLAGLLQPEAPRPWPLLCRLLQEVVLGMCYLH------SLDPPLLHRDLKPSNILLDPELHAKLADF---------------------GTLAYLDPELL---FKVN-LKASKASDVYSFGILVWAVLAGREAELV-------------D--------------QSRPPLTELPPGSPETPGLEKLKELMIHCW-------------------------------

General information: TITO was launched using: RESULT: Template: 6OKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 933 -26183 -28.06 -122.35 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -28.06 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_6OKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6oko-query.scw PDB file : Tito_Scwrl_6OKO.pdb: