Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGD-VAVKIFS--SREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLN----RYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI 3DQW Chain:B ((19-161)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKK--LRHEKLVQLYAVVSEEP-----IYIVIEYMSKGSLLDFLKGEMGKY-LRLPQLVDMAAQIASGMAYVE--------RMNYVHRDLRAANILVGENLVCKVADFGLA----------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DQW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 534 -18613 -34.85 -136.86 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -34.85 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.123

(partial model without unconserved sides chains): PDB file : Tito_3DQW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dqw-query.scw PDB file : Tito_Scwrl_3DQW.pdb: