Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGDVAVKIFS--SREERSWFREAEIYQTVMLRHENI---LGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGM--------IKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI 6N8G Chain:C ((29-232)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGEGGFGVVYKGYVNNTTVAVKKLADITTEELKQQFDQEIKVMAKCQHENLVELLGF-SSDGDD------LCLVYVYMPNGSLLDRLS----CLDGTPPLSWHMRCKIAQGAANGINFLH--------ENHHIHRDIKSANILLDEAFTAKISDFGLARASEK-AQVMRI-----VGTTAYMAPEALRGEITPK-------SDIYSFGVVLLEIITGLPAVDEHREPQL-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6N8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 872 10065 11.54 54.70 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.58 3D Compatibility (PKB) : 11.54 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.163

(partial model without unconserved sides chains): PDB file : Tito_6N8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6n8g-query.scw PDB file : Tito_Scwrl_6N8G.pdb: