Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKN-NMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEP-IYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSE-YTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP 2HYY Chain:B ((6-270)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKE-TMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYE-LMRACWQWNPSDRPSFAEIHQAFETMF-----------

General information: TITO was launched using: RESULT: Template: 2HYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1292 -44263 -34.26 -169.59 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -34.26 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_2HYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2hyy-query.scw PDB file : Tito_Scwrl_2HYY.pdb: