Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAPWVALALLWGSLCAGSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTLLTVLAYSLLPIGGLSLIVLLAFWMYRHRKPPYGHVDIHEDPGPPPPSPLVGLKPLQLLEIKARGRFGCVWKAQLMNDFVAVKIFPLQDKQSWQSEREIFSTPGMKHENLLQFIAAEKR-GSNLEVELWLITAFHDKGSLTDYLKGNIITWNELCHVAETMSRGLSYLHEDVPWCRGEGHKPSIAHRDFKSKNVLLKSDLTAVLADFGLAVRF-----EPGKPPGDTHGQVGTRRYMAPEVLEGAINFQRDAFLR-IDMYAMGLVLWELVSRCKAA--DGPVDEYMLPFEEEIGQHPSLEELQEVVVHKKMRPTIKDHWLKHP-GLAQLCVTIEECWDHDAEARLSAGCVEERVSLIRRSVNGTTSDCLVSLVTSVTNVDLPPKESSI 3G2F Chain:B ((10-317)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLKAYLSLHTSDWVSSCRLAHSVTRGLAYLHTELP--RGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTGNRL------------VGTIRYMAPEVLEGAVNLRDESALKQVDMYALGLIYWEIFMRCTDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEARLTAQAEERMAELM-------------------------------

General information: TITO was launched using: RESULT: Template: 3G2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1448 -36675 -25.33 -129.59 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -25.33 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_3G2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3g2f-query.scw PDB file : Tito_Scwrl_3G2F.pdb: