Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWRGGDVAVKIFS-SREERSWFREAEIYQTVMLRHENI---LGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTIEGM--------IKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI 4U9A Chain:A ((44-247)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGEGGFGVVYKGYVNNTTVAVKKLAA----LKQQFDQEIKVMAKCQHENLVELLGF-S-----------LCLVYVYMPNGSLLDRLS----CLDGTPPLSWHMRCKIAQGAANGINFLH--------ENHHIHRNIKSANILLDEAFTAKISDFGLA--------T--VMTSRIVGTTAYMAPEALRGEITPK-------SDIYSFGVVLLEIITGLPAVDEHREPQL-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4U9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 789 -16330 -20.70 -95.49 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -20.70 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_4U9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4u9a-query.scw PDB file : Tito_Scwrl_4U9A.pdb: