Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAILGDTIMV-------AKGLVKLTQAAVETHLQHLGIGGELIMAARALQSTAVEQ---IGMFLGKVQGQDKHEEYFAENFGGPEGEFHFSV-PHAAGA-STDFSSASAPDQSAPPSLGHAHSEGPAPAYVASGPFREAGFPGQASSPLGRANGRL-FANPRDSFSAMG------FQRRFFHQDQSPVGGLTAEDIEKARQAKARPENKQ-----HKQTLSEHARERKVPVTRIG--RLANFGGLAVGLGFGALAEVAKKSLRSEDPSGKKAVLGSSPFLSEANAERIVRTLCKVRGAALKLGQMLSIQDD----AFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAAS-IGQVHLARMKGGREVAMKIQYPGVAQSINSDVN---NLMAVLNMSNMLPEGLFPEHLIDVLRRELALECDY-QREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEIRNEICYNILVL---CLRELFEFHFMQTDPNWSNFFY-----DPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGYEVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLVPPPEETYS-------LHRKMGGSFLICSKLKARF--PCKAMFEEAYSNYCKRQAQQ-------- 5T0A Chain:A ((3-680)) ---IEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEI--TPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPP----KTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTF------KGQPSKPFVGVLSAGINAASPNKELAKEFLENY--LLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNI--PQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAKFNFTERDLTRDVDFNIKGDDVIVFLHIQKTGGTTFGRHLVRNIRLEQPCDCKAGQKKCTCHRPG----KQESWLFSRFSTGWSCGLHADWTELTNCVPVIMDRN---------FYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTQDELPTCYNGD----DWSGVTLHDFMDCPSNLANNRQVRMLA-DLSLVGCYNLSTMNESERNPILLASAKSNLKNMAFYGLTEFQRKTQYLFERTFHLRFISAFTQINSTRAANVELRDDMRSRIEQLNMLDMQLYEFAKDLFLQRYQFVRQRERQEERLKRREE

General information: TITO was launched using: RESULT: Template: 5T0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3227 107400 33.28 176.06 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 33.28 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_5T0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5t0a-query.scw PDB file : Tito_Scwrl_5T0A.pdb: