Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWR--GGDVAVKIFSSREERSWF-----REAEIYQTVMLRHENILGFIAADNKDNGTWTQ--LWLVSDYHEH---GSLFDYLNRYTVT-IEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQ--RVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI 3TN8 Chain:A ((26-201)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQ--LLKHENVVNLIEICRT-------GSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLN---GLYYIH--------RNKILHRDMKAANVLITRDGVLKLADFGLA---RAFSL----QPNRYNRVVTLWYRPPELL-----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TN8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 570 -8177 -14.34 -55.62 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -14.34 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.077

(partial model without unconserved sides chains): PDB file : Tito_3TN8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tn8-query.scw PDB file : Tito_Scwrl_3TN8.pdb: