Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACTSCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCCYTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRVYHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGRWR--GGDVAVKIFSSREERSWF-----REAEIYQTVMLRHENILGFIAADNKDNGTWTQ---LWLVSDYHEH---GSLFDYLNRYTVT-IEGMIKLALSAASGLAHLHMEIVGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQ--RVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI 3MIA Chain:A ((25-200)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQ--LLKHENVVNLIEICRT-------KGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLN---GLYYIH--------RNKILHRDMKAANVLITRDGVLKLADFGLA---RAFSLAKNSQPNRYNRVVTLWYRPPELL-----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3MIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 548 -572 -1.04 -3.79 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -1.04 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.089

(partial model without unconserved sides chains): PDB file : Tito_3MIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3mia-query.scw PDB file : Tito_Scwrl_3MIA.pdb: