Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSPRAVVQLGKAQPAGEELATANQTAQQPSSPAMRRLTVDDFEIGRPLGKGKFGNVYLARLK-ESHFIVALKVLFKSQIEKEGLEHQLRREIEIQAHLQHPNILRLYNYFHDARRVYLILEYAPRGELYKELQKSEKLDEQRTATIIEELADALTYCHDKKVIHRDIKPENLLLGF--RGEVKIADFGWSVH-TPSLRRKTMCGTLDYLPPEMIEGRTYDEKVDLWCIGVLCYELLVGYPPFESASHSETYRRILKVDVRFPLSMPL------GARDLISRLLRYQPLERLPLAQILKHPWVQAHSRRVLPPCAQMAS 6FDY Chain:U ((10-269)) ---------------------------------------LDGFILTERLG-----TVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLT-EIEILKGIRHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSMGVILYEALFGQPPFASRSFSELEEKIRSNRV---IELPLRPLLSRDCRDLLQRLLERDPSRRISFQDFFAHPWV----------------

General information: TITO was launched using: RESULT: Template: 6FDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain U - contact count / total energy / energy per contact / energy per residue : 1100 -58965 -53.60 -240.67 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain U : 0.76 3D Compatibility (PKB) : -53.60 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_6FDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6fdy-query.scw PDB file : Tito_Scwrl_6FDY.pdb: