Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSR-ERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFC-GTPEYLAPEVLEDND-----YGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFP-----RTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFD-EEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA 3QFV Chain:B ((75-415)) ----------------------------------------------------------------------------------------------------------------------------------------------------DFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIH-QLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFK---KHAFFEGLNWENI--RNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPP----------------------------

General information: TITO was launched using: RESULT: Template: 3QFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1569 -27817 -17.73 -93.34 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -17.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_3QFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3qfv-query.scw PDB file : Tito_Scwrl_3QFV.pdb: