Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSPRAVVQLGKAQPAGEELATANQTAQQPSSPAMRRLTVDDFEIGRPLGKGKFGNVYLARL---KESHFIVALKVLFKSQIEKEGLEHQLRREIEIQAHLQHPNILRLYNYFHDARRVYLILEYAPRGELYKELQKSEKLDEQRTATIIEELADALTYCHDKKVIHRDIKPENLLLGFRGEVKIADFGW---SVHTPSLRRKTMCGTLDYLPPEMIEGRTYDEKVDLWCIGVLCYELLVGYPPFESASHSETYRRILKVDVRFPLSMPLGARDLISRLLRYQPLERL-----PLAQILKHP------WVQAHSRRVLPPCAQMAS 3A60 Chain:B ((19-296)) ------------------------------------------FELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKK----------------------KHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKES-----------CGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPP------

General information: TITO was launched using: RESULT: Template: 3A60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 972 -10899 -11.21 -47.80 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -11.21 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_3A60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3a60-query.scw PDB file : Tito_Scwrl_3A60.pdb: