Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKN-NMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVT-KEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSE-YTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP 4XEY Chain:B ((7-385)) ----------------------------------------------------------------------------------------------------------------------LEKHSWYHGPVSRNAAEYLLSS--GINGSFLVRESESSPGQRSISLRYE----GRVYHYRINTASDGKLYV---SRFNTLAELVHHHSTVADGLITTLHYPAPK--------YDKWEMERTDITMKHKL------EVYEGVWKKYSLTVAVKTL---TMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVY-ELMRACWQWNPSDRPSFAEIHQAFETMFQES--------

General information: TITO was launched using: RESULT: Template: 4XEY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1792 -94743 -52.87 -269.15 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -52.87 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_4XEY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4xey-query.scw PDB file : Tito_Scwrl_4XEY.pdb: