Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLVLNDLLICCRQLEHDRATERKKEVEKFKRLIRDPETIKHLDRHSDSKQGKYLNWDAVFRFLQKYIQKETECLRIAKPNVSASTQASRQKKMQEISSLVKYFIKCANRRAPRLK-CQELLNYIMDTVKDSSNGAIYGADCSNILLKDILSVRKYW---CEISQQQWLELFSVYFRLYLKPSQDVHRVLVARIIHAVTKGCCSQTDGLNSKFLDFFSKAIQCAR-QE-KSSSGLNHILAALTIFLKTLAVNFRIRVCELGDEILPTLLYIWTQHRLNDSLKEVIIELFQLQIYIHH--PKGAKTQEKGAYESTKWRSILYNLYDLLVNEI-S--------HI--G-SR-GKYSSGFRNIAVKE---------NLIELMADICHQVFNEDTRSLEISQSYTTTQRESSDYSVPCKRKKIELGWEVIKDHLQKSQNDFDLVPWLQIATQLISKYPASLPNCELSPLLMILSQLLPQQRHGERTPYVLRCLTEVALCQDKRSNLESSQKSDLLKLWNKIWCITFRGISSEQIQAENFGLLGAIIQGSLV------EVDREFWK-LFTGSACRPSCPAVCCLTLALTTSIVPGTVKMGIEQNMCEVN-RSFSLKESIMKWLLFYQLEGDLENSTEVPPILHSNFPHLVLE---KILVSLTMKNCKAAMNFFQSVPECEH-HQKDKEELSFSEVEELFLQTTFDKMDFLTIVRECGIEKHQSSIG-FSVHQNLKESLDRCLL---GLSEQLLNNYSSEITNSETLVRCSRLLVGVLGCYCYMG--VIAEEEAYKSELFQKAKSLMQCAGESITLFKNKTNEEFRIG-SLRNMMQLCTRCLSNCTKKSPNKIASGFFLRLL-T----SKLMNDIADICKSLASFIKKPFDRGEVESMEDDTNGNLMEVEDQSSMNLFNDYPDSSVSDANEPGESQSTIGAINPLAE-EYLSKQDLLFLDML---KFLCLCVTTAQTNTVSFRAADIRRKLLMLIDSSTLEPTKSLHLHMYLMLLKELPGEEYPLPMEDVLELLKPLSNVCSLY--RRDQDVCKTI---LNHVLHVVKNLGQSNMDSENTRDAQGQFLTVIGAFWH-LTKERKYIFSVRMALVNCLKTLLEADPYSKWAILNVMGKDFPVNEVFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQQTAFENAYLKAQEGMREMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFALCKSVKENG---LEPHLVKKVLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSFPFILLNYTNIEDFYRSCYKVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVNILPYFAYEGTRDSGMAQQRETATKVYDMLKSENLLGKQ-IDHLFISNLPEIVVELLMTLHEPANSSA----SQ--------STDLCDFS--GDLDPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKSPDSYQKILLAICEQAAETNNVYKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIYTLIHYINQRPSCIMDVSLRSFSLCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVYEQV---E--------VQKQVLDLLKYLVIDNKDNENLYITIKLLDPFP-----DHVVFKDLRITQQKIKYSRGPFSLL-EEINHFLSVSVYDALPLTRLEGLKDLRRQLELHKDQM--VDIMRAS-----------QDNPQDGIMVKLVVNLLQLSKMAINHTGEKEVLEAVGSCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDKELQWTFIMLTYLNNTLVEDCVKVRSAAVTCLKNILATKTGHS---FWEIYKMTTDPMLAYLQP-----FRTSRKKFLEVPRFDKENPFEGLDDINLWIPLSENHDIWIKTLTCAFLDSGGTKCEILQLLKPMCEVKTDFCQTVLPYLIHDILLQDTN--ESWRNLLSTHVQGFFTSCLRHFSQTSRSTTPANLDSESEHFFRCCLDKKSQRTMLAVVDYMRRQKRPSSGTIFNDAFWLDLNYLEVAKVAQSCAAHFTALLYAEIYADKKSMDDQEKRSLAFEEGSQSTTISSLSEKSKEETGISLQDLLLEIYRSIGEPDSLYGCGGGKMLQPITRLRTYEHEAMWGKALVT----YDLETAIPSS---TRQAGIIQALQNLGLCHILSVYLKGLDYENKDWCPELEELHYQAAWRNMQWDHCTSVSKEVEGTSYHESLYNALQSLRDR-EFSTFYESLKYARVKEVEEMCKRSLESVYSLYPTLSRLQAIGELESIGELFSRSVTHRQLSEVYIKWQKHSQLLKDSDFSFQEPIMALRTVILEILMEKEMDNS---QRECIKDILTKHLVELSILARTFKNTQLPERAIFQIKQYNSVSC-----GVSEWQLEEAQVFWAKKEQSLALSILKQMIKKLDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSDELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRS--RRPQMVRSVEALCDAYIILANLDATQ-WKTQRKGIN---IPA-DQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKG-RDDLRQDAVMQQVFQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGGQVNLLIQQAIDPKNLSRLFPGWKAWV 6SL1 Chain:A ((14-2944)) -------FKEIESHLV--SATARKDAVEALIAYF---------------------AYHRLFEALFRCTLIEKEAY------------AAAAARLERCPEALRLAVRHGVTT-IRRKTARAIIDHIVQVLP-----MPLLAGYVKV-LYEFLDNPASAENIAALSGEGWEVCVDFCIDVLSRF----GTHVAVDVLSCLYMLCIAHNAPIQRKADRLPHVVIQLLQLRQMKIGELQKMAFATFNIVFQRMQAEDVALCKTLVKQVVPLLSHWW----MLNSIRDEMLKTLYGTRLYIQALLRE---ESF-PQDV---EELLDTLWCDYSRREERARLQLDDITFTNMLLPPDHPRT--GIFSLRPHHTAGEQNWALLENLAILEAAYSKHG---------------------------------NRVHQKL-HSLDPAVRLSALQLIPFLTRHKKPSLE--DVAETLEDLSKHVTAKQ-AIVASWAMLACSSLAIHEVSRH-------PSLSSSWKQLWQLAVRSLSLPPISRASCVLLNSILKANLIPRHELADDINQIVTTADISGPAILVDASLGLMLNLLRFRNN-------------MFPNASQATSNHIIRWVFVRWSPAELTY-AS----LHG--THATPYDLVNLLRACYGISPLVMAQ-PLRLFNGPIVLHWKEQ-AEMEPF-IRYLLLL-----------------AGSNASRRLALELFYPKVEELQELAESW--------SMERLRSM--VLACLTGALLLPDLVNINSSLSRDLESAVFSIVDATLKV--------------ILNS-PPSENLFGMILAS---SAPYI---P-HLIEPELIALKRERPHLLKFFGKLSEALYERSRR--------------------------------------------------------TLPRRDILLSYTPEAFYLETSLRIHFLDIIRLND--GEIGRIPEPIINQLAG------LSGEQFLC---CREFMREIFTSDAIVPLGGATTILETAGHIVSRYEYACCEVALCNCIDIMDSFINLWTDNH---------FDIAEMAGDLYHYLVKQSLPNNSMSAAAQIRLASLLLHLLEVKSEYAS-----NLGLPSSQSTLLKILQDGPMKLKHYIGLEIPKLFGLYVLK---------------THDDIFVDVLEHL-P--SDP-------DVVEGLAFRLFVLAELACRWPTLLRRSIYHTFEIPG---CVTHSALYAASCIKRIAQTLKLSGPQELFKLFAPQLLYTWLDN---D-SIQDIAYEIFGFSSLLDLLREAQTEAAAIMMMRGQEQEVCQLAQSLGLTPEKLVQQSFTKIIAYSIAHDISIA------------YVTGESRMR--KILGKEEYLANIHLNFADIISTFFDIFDQEDPIEKAFRRDERFAYAAETLEEIKKLGHLPTALPPNQQPMFRAKYLPREIVHLCSRTQYEPENI-----WTPALVVFVARKLLKTIHPALGPLHACSVLRKIRVLICLAGDHAIS------GYPLEMLLHSLRVFVVDP-------------ECADDALGITQYLIKRGDEYLKRTPSFLAGYALSSLADLRVFLSSQESQFKATKSKAQEFHAWFS----------KYLAAYDSPEFKDEGQKQAFRSITENAAHIRAS-GNAEKGTHESNLLLEILK-DW----GR-----ENQLLNEPARDVALSMLCGVFNIPPSSRLDVIETDEDAIKNGAVVWKSCSSQRL----G--GEYLAWAGRVLGRSFAASGEVPEDLLRESQ-LQE-YRRLSQGVGSSEEGLLNLIKSLTISGDCFTAGLAEAALRTIVSDAISDNDHDLLSACQE--SLPEPLLIASNWDPYRTPLSD-------------------EVFSARALENPNWSQHLAIRLALSAP-KIVTLRVLPPILSKVKGFAERAFPFVVHLVLAYQLDKQQSAKRELSESLQEWLNFTS-------------------------EPAKENLKLLINTILYLRTQPLPGESSIADRAHWLDVNMASAAAAATRCGMYKVALLFAELAAESTR-----------------------------------SDILLEIFENIDDPDAYYGLSQDASL--STVLARLEYENDGAKSLAFRGAQYDSHLRGRDLQSRQDCNALIKALSSLGLAGLSNSLLQSQ----------SLDATFTTARKLEIWNLPAPVN----SDSWAVTVYKAYQSMYQAQELDTVRSMVHDGLKNTVRHLSSGS-LNTSVLRQQLGALAALTELDDILNVRD----QSELQCTLATFEKRSKWMMSGRYADVSQILSCRETTLSMWSQRHNLRAAGLTSADARLVQIRGMLLSSDIFRFHRARQETLNLSTALSDLIPSCESLGLSVDAAIKMEAANALWDHGEMISSIRMLQAIDKDSSLKK----QSVPLSRSDLLSKIGYQVSVARLESPDAIQKKYLEPALKELKGKIE------GREAGQVFHQFAVFCDEQLQNPD---SLEDLARLQNLKKGKDEEVAQLK-------SHLAKAKQWQELDQQELRRVEQTRSEFLKLCIENYLLSLAASDEHDNDALRFMALWLEKSEEEVANEVVKKWINKVPTRKFALLMNQLSSRLQDHN---TLFQKLLIDLVYRICVDHPYHGMYHIWTGARTRVNKD--------------------DEVAVSRQRATDKIAKALSKNNKVSSIWPAIDQTSRVYHALAMDRDPTRYKSG-QKVPIKNSPVGQNFLSTMSN-NPIPPPTLQIEVSANLDYSHVPMIHKFAPEMAIASGVSAPKILTAIGTDGRKYKQLVKGGNDDLRQDAIMEQVFAAVSELLKLHRETRQRNLGIRTYKVLPLTSSSGLIEFVSNTIPLHEYLMP----AHERYYPKDLKGSQCRKEIANAQTKNTETRIAVYRRVTERFHPVMRYFFMEYFPDPDEWFQKRTNYTRTTAAISMLGHVLGLGDRHGHNILLDHKTGEVVHIDLGVAFEMGRVLPVPELVPFRLTRDIVDGMGITKTEGVFRRCCEFTLDALREEAASIQTILDSLRHDTLYQWSISPVRMAKLQN-------------------------------SEADRAIEVVKKKL-----SKTLSVMATVNDLINQATSVSNLAVLYSGWAAYA

General information: TITO was launched using: RESULT: Template: 6SL1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 12136 -203616 -16.78 -81.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -16.78 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_6SL1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6sl1-query.scw PDB file : Tito_Scwrl_6SL1.pdb: